![]() The spectral contrast image demonstrates how blood vessels are highlighted by a shadow and tissue is ignored. In images from the inverse OCT intensity at 557 nm, hereafter referred to as inverse 557 nm images, blood vessels can easily be seen due to the high contrast and high absorption provided in the visible range. 1b) of lower human labial mucosa (inner side of lip) can be seen with the ratio of the OCT image intensities from the two Kaiser windows (620 nm divided by 557 nm), hereafter referred to as SC-OCTA. ![]() d, e The white arrow shows a salivary duct that is correctly not identified by SC-OCTA in e The red box shows a blow-up of the capillary loop scale bar 20 µm. Line 1 (L1) and Line 2 (L2) are line profiles in Figure S3 for comparison with the simulated line profile results. e Depth-encoded vessel map of the same FOV as d with saturation and value from SC-OCTA and hue from the depth of the vessel in inverse 557 nm. Depth ranges were chosen to maximize the contrast of the en face projections for different techniques. c Comparison of angiography en face projections of superficial capillary loops with traditional motion contrast OCTA (64–111 µm), inverse 557 nm (55.6–140 µm), and SC-OCTA (83–209 µm) with their corresponding line profile intensities. b Inverse 557 nm and inverse 620 nm B-scans with their corresponding STFT windows, as well as a SC-OCTA B-scan showing contrast shadows from each vessel. b– e In vivo human imaging of labial mucosa (lower lip) from a healthy volunteer. a Simplified schematic of the visible OCT system, which allowed 3D spectral information of the sample to be obtained.
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